Stefan Krauss

Dr. Stefan Krauss– Director
Unit for Cell Signaling
Dept. of Microbiology
Oslo University Hospital,
Rikshospitalet
stefan.krauss@rr-research.no
Tel +47 23 07 90 17

 


 

ACTIVITIES

Since its discovery in 1993 (Krauss et al., Cell 75, 1431-1444) Shh has shown to be a central signal in numerous developmental systems. The laboratory for cell signalling focuses on Hh and Wnt/beta-catenin signalling in development, stem cells, and tumors. The laboratory currently works on pathways that feed into Hh and Wnt/beta-catenin signaling. Using chemical biology, the laboratory develops novel Hh and Wnt/beta-catenin.


GROUP MEMBERS                                 

Andrey Voronkov
Post. Doc.

Jo Waaler
PhD student

Line Mygland
Engineer/Administrator

Nina Solberg
Post Doc.

Petter A. Olsen
Senior Scientist

Collaborative members with Elsa Lundanes/Tyge Greibrokk/Steven Wilson -group;

Hanne Røberg-Larsen
PhD student

Tore Vehus
PhD student

Dorna Misaghian
Engineer

Other Collaborative members include;

Martin Strand
Post. Doc.

Kaja Lund
PhD. student

 

STATUS 2013

Wnt/β-catenin signaling is a functional network that degulates central stem cell properties in a cell and broad range of biological systems, including organ development and control of intracellular metabolism. Deregulated canonical Wnt signaling is a common denominator in a variety of tumors. We have identified the adenosine binding pocket of tankyrase as an attractive biotarget to inhibit Wnt/β-catenin signaling. We have developed a series of highly specific tankyrase inhibitors that show efficacy in vitro in various tumor cell lines, and in vivo in mousexenografts. The inhibitors are now tested with the aim of using the inhibitors as potential human therapeutics (Waaler et al., 2011; Waaler et al., 2012; Voronkov et al., 2013; Lau et al., 2013).

 

SELECTED PUBLICATIONS

Lehtiö LChi NWKrauss S (2013)
Tankyrases as drug targets
FEBS J280 (15)3576-93
PubMed 23648170

Lau TChan ECallow MWaaler JBoggs JBlake RAMagnuson SSambrone ASchutten MFirestein RMachon OKorinek VChoo EDiaz DMerchant MPolakis PHolsworth DDKrauss SCosta M (2013)
A novel tankyrase small-molecule inhibitor suppresses APC mutation-driven colorectal tumor growth
Cancer Res73 (10)3132-44
PubMed 23539443

Voronkov AHolsworth DDWaaler JWilson SREkblad BPerdreau-Dahl HDinh HDrewes GHopf CMorth JPKrauss S (2013)
Structural basis and SAR for G007-LK, a lead stage 1,2,4-triazole based specific tankyrase 1/2 inhibitor
J Med Chem56 (7)3012-23
PubMed 23473363

Solberg NKrauss S (2013)
Luciferase assay to study the activity of a cloned promoter DNA fragment
Methods Mol Biol97765-78
PubMed 23436354

Voronkov AKrauss S (2013)
Wnt/beta-catenin signaling and small molecule inhibitors
Curr Pharm Des19 (4)634-64
PubMed 23016862

Roberg-Larsen HStrand MFGrimsmo AOlsen PADembinski JLRise FLundanes EGreibrokk TKrauss SWilson SR (2012)
High sensitivity measurements of active oxysterols with automated filtration/filter backflush-solid phase extraction-liquid chromatography-mass spectrometry
J Chromatogr A1255291-7
PubMed 22410154

Solberg NMachon OMachonova OKrauss S (2012)
Mouse Tcf3 represses canonical Wnt signaling by either competing for β-catenin binding or through occupation of DNA-binding sites
Mol Cell Biochem365 (1-2)53-63
PubMed 22270545

Waaler JMachon OTumova LDinh HKorinek VWilson SRPaulsen JEPedersen NMEide TJMachonova OGradl DVoronkov Avon Kries JPKrauss S (2012)
A novel tankyrase inhibitor decreases canonical Wnt signaling in colon carcinoma cells and reduces tumor growth in conditional APC mutant mice
Cancer Res72 (11)2822-32
PubMed 22440753

Solberg NMachon OKrauss S (2012)
Characterization and functional analysis of the 5'-flanking promoter region of the mouse Tcf3 gene
Mol Cell Biochem360 (1-2)289-99
PubMed 21935611

Jing YMachon OHampl ADvorak PXing YKrauss S (2011)
In vitro differentiation of mouse embryonic stem cells into neurons of the dorsal forebrain
Cell Mol Neurobiol31 (5)715-27
PubMed 21424551

Strand MFWilson SRDembinski JLHolsworth DDKhvat AOkun IPetersen DKrauss S (2011)
A novel synthetic smoothened antagonist transiently inhibits pancreatic adenocarcinoma xenografts in a mouse model
PLoS One6 (6)e19904
PubMed 21698280

Irvine EEDrinkwater LRadwanska KAl-Qassab HSmith MAO'Brien MKielar CChoudhury AIKrauss SCooper JDWithers DJGiese KP (2011)
Insulin receptor substrate 2 is a negative regulator of memory formation
Learn Mem18 (6)375-83
PubMed 21597043

Waaler JMachon Ovon Kries JPWilson SRLundenes EWedlich DGradl DPaulsen JEMachonova ODembinski JLDinh HKrauss S (2011)
Novel synthetic antagonists of canonical Wnt signaling inhibit colorectal cancer cell growth
Cancer Res71 (1)197-205
PubMed 21199802

Kalousova AMavropoulos AAdams BANekrep NLi ZKrauss SStainier DYGerman MS (2010)
Dachshund homologues play a conserved role in islet cell development
Dev Biol348 (2)143-52
PubMed 20869363

Dembinski JLKrauss S (2010)
A Distinct Slow-Cycling Cancer Stem-like Subpopulation of Pancreatic Adenocarcinoma Cells is maintained in Vivo
Cancers2 (4)2011-2025
PublikaID 116

Wilson SRStrand MFKrapp ARise FHerstad GMalterud KEKrauss S (2010)
Hedgehog antagonists cyclopamine and dihydroveratramine can be mistaken for each other in Veratrum album
J Pharm Biomed Anal53 (3)497-502
PubMed 20646889

Wilson SRStrand MFKrapp ARise FPetersen DKrauss S (2010)
Hedgehog antagonist cyclopamine isomerizes to less potent forms when acidified
J Pharm Biomed Anal52 (5)707-13
PubMed 20236786

Olsen PAGelazauskaite MRandøl MKrauss S (2010)
Analysis of illegitimate genomic integration mediated by zinc-finger nucleases: implications for specificity of targeted gene correction
BMC Mol Biol1135
PubMed 20459736

Kunke DBryja VMygland LArenas EKrauss S (2009)
Inhibition of canonical Wnt signaling promotes gliogenesis in P0-NSCs
Biochem Biophys Res Commun386 (4)628-33
PubMed 19545542

Hildrestrand GANeurauter CGDiep DBCastellanos CGKrauss SBjørås MLuna L (2009)
Expression patterns of Neil3 during embryonic brain development and neoplasia
BMC Neurosci1045
PubMed 19426544

Dembinski JLKrauss S (2009)
Characterization and functional analysis of a slow cycling stem cell-like subpopulation in pancreas adenocarcinoma
Clin Exp Metastasis26 (7)611-23
PubMed 19421880

Olsen PASolhaug ABooth JAGelazauskaite MKrauss S (2009)
Cellular responses to targeted genomic sequence modification using single-stranded oligonucleotides and zinc-finger nucleases
DNA Repair (Amst)8 (3)298-308
PubMed 19071233

Faedo ATomassy GSRuan YTeichmann HKrauss SPleasure SJTsai SYTsai MJStuder MRubenstein JL (2008)
COUP-TFI coordinates cortical patterning, neurogenesis, and laminar fate and modulates MAPK/ERK, AKT, and beta-catenin signaling
Cereb Cortex18 (9)2117-31
PubMed 18165280

Solberg NMachon OKrauss S (2008)
Effect of canonical Wnt inhibition in the neurogenic cortex, hippocampus, and premigratory dentate gyrus progenitor pool
Dev Dyn237 (7)1799-811
PubMed 18521945

Machon OBackman MMachonova OKozmik ZVacik TAndersen LKrauss S (2007)
A dynamic gradient of Wnt signaling controls initiation of neurogenesis in the mammalian cortex and cellular specification in the hippocampus
Dev Biol311 (1)223-37
PubMed 17916349

Hildrestrand GADiep DBKunke DBolstad NBjørås MKrauss SLuna L (2007)
The capacity to remove 8-oxoG is enhanced in newborn neural stem/progenitor cells and decreases in juvenile mice and upon cell differentiation
DNA Repair (Amst)6 (6)723-32
PubMed 17236821

Kreslova JMachon ORuzickova JLachova JWawrousek EFKemler RKrauss SPiatigorsky JKozmik Z (2007)
Abnormal lens morphogenesis and ectopic lens formation in the absence of beta-catenin function
Genesis45 (4)157-68
PubMed 17410548

Kadowaki MNakamura SMachon OKrauss SRadice GLTakeichi M (2007)
N-cadherin mediates cortical organization in the mouse brain
Dev Biol304 (1)22-33
PubMed 17222817

Fujimura NVacik TMachon OVlcek CScalabrin SSpeth MDiep DKrauss SKozmik Z (2007)
Wnt-mediated down-regulation of Sp1 target genes by a transcriptional repressor Sp5
J Biol Chem282 (2)1225-37
PubMed 17090534