Elsa Lundanes / Tyge Greibrokk / Steven Wilson

 

Dr. Elsa Lundanes
Analytical Chemistry
Dept. of Chemistry
University of Oslo
elsa.lundanes@kjemi.uio.no
Tlf: +47 22 85 55 53

Dr. Tyge Greibrokk
Analytical Chemistry
Dept. of Chemistry
University of Oslo
tyge.greibrokk@kjemi.uio.no
Tlf: +47 22 85 50 27
            

 

Dr. Steven Wilson
Analytical Chemistry
Dept. of Chemistry
University of Oslo
stevenw@kjemi.uio.no

 

 


 

ACTIVITIES

We are developing highly sensitive and selective capillary LC-MS methods for potential novel drugs in different types of organs as liver, kidney, plasma etc, in cooperation with Stefan Krauss and his group, in order to determine if the drugs reach the target organs and for pharmacokinetic studies.

We are also developing miniaturized liquid chromatography methods for identification of proteins, especially membrane proteins, in cooperation with Iver Langmoen and his group. Novel separation technology in the nano-flow region will be explored for the ability to separate the hydrophobic membrane proteins.

On-line multidimensional capillary liquid chromatographic methods with mass spectrometric detection of peptides have already been developed for identification of the proteins.

 

GROUP MEMBERS
a
ctive in CAST 

Hanne Rødberg-Larsen
Post doc

Helle Malerød
Post doc

Tore Vehus
PhD student


STATUS 2013

The Lundanes-group has developed analytical tools and methodology for sensitive determination of metabolites which may be potential cancer biomarkers, such as oxysterols. The methodology is currently being used to study interplay between central signal pathways such as Wnt and Hh.

The Lundanes-group is currently also focusing on mass spectrometric methodology combined with in-house synthesized chromatography columns. Application areas are development of targeted proteomics for accurate and precise determination of central proteins in Wnt and Hh, as well as post translational modification analysis.

 

SELECTED PUBLICATIONS

Rogeberg M, Vehus T, Grutle L, Greibrokk T, Wilson SR, Lundanes E. (2013)
Separation optimization of long porous layer open tubular columns for nano-LC-MS of limited proteomic samples.
J Sep Sci. (in press)
PubMed 
23813982 

Røen BTSellevåg SRLundanes E. (2013)
On-line solid phase extraction-liquid chromatography-mass spectrometry for trace determination of nerve agent degradation products in water samples.
Anal Chim Acta. 25;761, 109-16.
PubMed 23312321 

Roberg-Larsen H, Strand MF, Grimsmo A, Olsen PA, Dembinski JL, Rise F, Lundanes E, Greibrokk T, Krauss S, Wilson SR. (2012)
High sensitivity measurements of active oxysterols with automated filtration/filter backflush-solid phase extraction-liquid chromatography-mass spectrometry.
J Chromatogr A. 14;1255, 291-7
PubMed 22410154

Svendsen KOLarsen HRPedersen SABrenna ILundanes EWilson SR. (2011)
Automatic filtration and filter flush for robust online solid-phase extraction liquid chromatography.
J Sep Sci. 34(21), 3020-2
PubMed 21919201

Rogeberg M, Wilson SR, Malerod H, Lundanes E, Tanaka N, Greibrokk T. (2011)
High efficiency, high temperature separations on silica based monolithic columns.
J Chromatogr A. 1218(41), 7281-8
PubMed 21899856

Hustoft HK, Reubsaet L, Greibrokk T, Lundanes E, Malerod H. (2011)
Critical assessment of accelerating trypsination methods.
J Pharm Biomed Anal. 56(5), 1069-78
PubMed 
21873015

Solbu K, Hersson M, Thorud S, Lundanes E, Nilsen T, Synnes O, Ellingsen D, Molander P. (2010)
Compact semi-automatic incident sampler for personal monitoring of volatile organic compounds in occupational air.
J Environ Monit. 12(5):1195-202.
PubMed 21491688

Solbu KDaae HLOlsen RThorud SEllingsen DGLindgren TBakke BLundanes EMolander P. (2011)
Organophosphates in aircraft cabin and cockpit air--method development and measurements of contaminants.
J Environ Monit. 13(5), 1393-403
PubMed 21399836

Johnsen E, Wilson SR, Odsbu I, Krapp A, Malerod H, Skarstad K, Lundanes E. (2011)
Hydrophilic interaction chromatography of nucleoside triphosphates with temperature as a separation parameter.
J Chromatogr A. 1218(35), 5981-6
PubMed 21315360

Solbu K, Daae HL, Thorud S, Ellingsen DG, Lundanes E, Molander P. (2010)
Exposure to airborne organophosphates originating from hydraulic and turbine oils among aviation technicians and loaders.
J Environ Monit. 12(12), 2259-68
PubMed 20949195

Rogeberg M, Wilson SR, Greibrokk T, Lundanes E. (2010)
Separation of intact proteins on porous layer open tubular (PLOT) columns.
J Chromatogr A. 1217(17), 2782-6
PubMed 20299025

Røen BTUnneberg ETørnes JALundanes E. (2010)
Headspace-trap gas chromatography-mass spectrometry for determination of sulphur mustard and related compounds in soil.
J Chromatogr A. 1217(14), 2171-8
PubMed 20189185

Loftheim H, Nguyen TD, Malerød H, Lundanes E, Asberg A, Reubsaet L. (2010)
2-D hydrophilic interaction liquid chromatography-RP separation in urinary proteomics--minimizing variability through improved downstream workflow compatibility.
J Sep Sci. 33(6-7), 864-72
PubMed 20024930

Røen BTUnneberg ETørnes JALundanes E (2010)
Trace determination of sulphur mustard and related compounds in water by headspace-trap gas chromatography-mass spectrometry.
J Chromatogr A. 1217(5), 761-7
PubMed 
20015497

Berg T, Lundanes E, Christophersen AS, Strand DH. (2009)
Determination of opiates and cocaine in urine by high pH mobile phase reversed phase UPLC-MS/MS.
J Chromatogr B Analyt Technol Biomed Life Sci. 877(4), 421-32
PubMed 19144579

Tran BQ, Loftheim H, Reubsaet L, Lundanes E, Greibrokk T. (2008)
On-Line multitasking analytical proteomics: how to separate, reduce, alkylate and digest whole proteins in an on-Line multidimensional chromatography system coupled to MS.
J Sep Sci. 31(16-17), 2913-23
PubMed 18704998

Dahl SR, Kleiveland CR, Kassem M, Lea T, Lundanes E, Greibrokk T (2008)
Detecting pM concentrations of prostaglandins in cell culture supernatants by capillary SCX-LC-MS/MS.
J Sep Sci. 31(14), 2627-33
PubMed 18618469

Olsen R, Ovrebø S, Thorud S, Lundanes E, Thomassen Y, Greibrokk T, Molander P. (2008)
Sensitive determination of a glyoxal-DNA adduct biomarker candidate by column switching capillary liquid chromatography electrospray ionization mass spectrometry.
Analyst. 133(6), 802-9
PubMed 
18493683

Mihailova A, Malerød H, Wilson SR, Karaszewski B, Hauser R, Lundanes E, Greibrokk T. (2008)
Improving the resolution of neuropeptides in rat brain with on-line HILIC-RP compared to on-line SCX-RP.
J Sep Sci. 31(3), 459-67
PubMed 18266260

Mihailova A, Karaszewski B, Faergestad EM, Hauser R, Nyka WM, Lundanes E, Greibrokk T. (2008)
Two-dimensional LC-MS/MS in detection of peptides in hypothalamus of the rat subjected to hypoxic stress.
J Sep Sci. 31(3), 468-79
PubMed 18210376

Mmatli EE, Malerød H, Wilson SR, Abegaz B, Greibrokk T, Lundanes E, Malterud KE, Petersen D, Rise F. (2007)
Identification of major metal complexing compounds in Blepharis aspera.
Anal Chim Acta. 597(1), 24-31
PubMed 17658309

Rinne SRamstad Kleiveland CKassem MLea TLundanes EGreibrokk T. (2007)
Fast and simple online sample preparation coupled with capillary LC-MS/MS for determination of prostaglandins in cell culture supernatants.
J Sep Sci. 30(12), 1860-9
PubMed 17638370

Olsen R, Thorud S, Hersson M, Ovrebø S, Lundanes E, Greibrokk T, Ellingsen DG, Thomassen Y, Molander P. (2007)
Determination of the dialdehyde glyoxal in workroom air-development of personal sampling methodology.
J Environ Monit. 9(7), 687-94
PubMed 17607389

Olsen R, Backman J, Molander P, Øvrebø S, Thorud S, Lundanes E, Greibrokk T, Kronberg L. (2007)
Characterization of adducts formed in the reaction of glutaraldehyde with 2'-deoxyadenosine.
Chem Res Toxicol. 20(6), 965-74
PubMed 17518482

Wilson SR, Malerød H, Holm A, Molander P, Lundanes E, Greibrokk T. (2007)
On-line SPE-Nano-LC-Nanospray-MS for rapid and sensitive determination of perfluorooctanoic acid and perfluorooctane sulfonate in river water.
J Chromatogr Sci. 45(3), 146-52
PubMed 17462129

Bjellaas TOlesen PTFrandsen HHaugen MStølen LHPaulsen JEAlexander JLundanes EBecher G. (2007)
Comparison of estimated dietary intake of acrylamide with hemoglobin adducts of acrylamide and glycidamide.
Toxicol Sci. 98(1), 110-7
PubMed 17449897

Wilson SR, Malerød H, Petersen D, Rise F, Lundanes E, Greibrokk T. (2007)
An alternative multiple-trapping LC-SPE-NMR system.
J Sep Sci. 30(3), 322-8
PubMed 17396589

Mihailova A, Karaszewski B, Hauser R, Lundanes E, Greibrokk T. (2007)
Identification of neuropeptides in rat brain rhinencephalon.
J Sep Sci. 30(2), 249-56
PubMed 17390620

Bjellaas TStølen LHHaugen MPaulsen JEAlexander JLundanes EBecher G.(2007)
Urinary acrylamide metabolites as biomarkers for short-term dietary exposure to acrylamide.
Food Chem Toxicol. 45(6), 1020-6
PubMed 17258374

Kristoffersen L, Øiestad EL, Opdal MS, Krogh M, Lundanes E, Christophersen AS. (2007)
Simultaneous determination of 6 beta-blockers, 3 calcium-channel antagonists, 4 angiotensin-II antagonists and 1 antiarrhythmic drug in post-mortem whole blood by automated solid phase extraction and liquid chromatography mass spectrometry. Method development and robustness testing by experimental design.
J Chromatogr B Analyt Technol Biomed Life Sci. 850(1-2), 147-60
PubMed

Ioan I, Wilson S, Lundanes E, Neculai A. (2007)
Comparison of Fenton and sono-Fenton bisphenol A degradation.
J Hazard Mater. 142(1-2), 559-63
PubMed 17011123


Steven Ray Wilson's Science Page

Elsa Lundanes/Tyge Greibrokk/Steven Wilson laboratory